| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CTBP1; CTBP; C-terminal-binding protein 1; CtBP1 |
| Entrez GeneID | 1487 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human CtBP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CTBP1抗体的参考文献示例(注：文献为虚构示例，仅作格式参考)：
1. **"CTBP1 modulates HIF-1α stability in hypoxic cancer cells"**
- **作者**: Smith A, et al.
- **摘要**: 研究通过Western blot(使用CTBP1抗体)和免疫共沉淀技术，揭示了CTBP1在缺氧条件下与HIF-1α相互作用并调控其稳定性的机制，提示其在肿瘤代谢中的潜在作用。
2. **"CTBP1-mediated transcriptional repression in epithelial-mesenchymal transition"**
- **作者**: Lee J, et al.
- **摘要**: 利用CTBP1抗体进行免疫组化分析，发现CTBP1在转移性乳腺癌组织中高表达，并通过抑制E-cadherin等基因促进肿瘤细胞的侵袭转移。
3. **"Subcellular localization of CTBP1 in neuronal development"**
- **作者**: Garcia R, et al.
- **摘要**: 通过免疫荧光(CTBP1抗体标记)和蛋白质分馏实验，证实CTBP1在神经元分化过程中从细胞核转位至细胞质，调控突触形成相关基因的表达。
4. **"CTBP1 as a metabolic sensor: Role in lipid biosynthesis"**
- **作者**: Wang Y, et al.
- **摘要**: 研究使用CTBP1抗体进行ChIP-seq和功能实验，发现CTBP1通过结合脂质合成酶基因启动子区域，响应NADH/NAD+比例变化并调控代谢重编程。
(注：以上文献及作者为模拟生成，实际研究中建议通过PubMed或Web of Science检索真实文献。)
**Background of CTBP1 Antibody**
CTBP1 (C-terminal Binding Protein 1) is a transcriptional corepressor that regulates gene expression by interacting with DNA-binding proteins and chromatin-modifying enzymes. It plays a critical role in cellular processes, including apoptosis, differentiation, and oncogenesis. CTBP1 binds to the C-terminal region of adenovirus E1A proteins and various transcription factors (e.g., EZH2. ZEB1), recruiting histone deacetylases (HDACs) or other repressive complexes to silence target genes. Its activity is modulated by NAD+/NADH levels, linking cellular metabolism to epigenetic regulation.
CTBP1 antibodies are essential tools for studying its expression, localization, and function in normal and disease contexts, particularly cancer. Overexpression of CTBP1 is associated with tumor progression, metastasis, and therapy resistance, making it a potential therapeutic target. These antibodies are used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect CTBP1 protein levels, assess interactions with binding partners, or map its genomic occupancy.
Commercial CTBP1 antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and validated for cross-reactivity, specificity (e.g., distinguishing CTBP1 from CTBP2), and performance in relevant samples. Researchers should verify antibody suitability for their experimental models, as post-translational modifications or splice variants may affect detection.
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