首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织蛋白酶H (CTSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSH含量。CTSH即组织蛋白酶H，它属于半胱氨酸蛋白酶家族。在人体生理功能维持上扮演重要角色。研究聚焦其在肿瘤发生发展中的作用机制，包括促进肿瘤细胞增殖、迁移和侵袭等，还探索其在神经系统疾病等领域的潜在病理机制，为相关疾病治疗提供新方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可利用该产品开展CTSH在肿瘤发生发展、免疫细胞功能调控、神经退行机制等领域的表达特征研究，也可用于药物干预实验中生物标志物的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACC 4 ELISA Kit; ACC 5 ELISA Kit; ACC4 ELISA Kit; ACC5 ELISA Kit; aleurain ELISA Kit; CATH_HUMAN ELISA Kit; cathepsin B3 ELISA Kit; cathepsin BA ELISA Kit; Cathepsin H heavy chain ELISA Kit; Cathepsin H light chain ELISA Kit; Cathepsin H mini chain ELISA Kit; CPSB ELISA Kit; CTSH ELISA Kit; minichain ELISA Kit; N benzoylarginine beta naphthylamide hydrolase ELISA Kit; N-benzoylarginine-beta-naphthylamide hydrolase ELISA Kit; pro cathepsin H ELISA Kit
缩写：CTSH
Uniprot No.：P09668
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 89-106 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 82-97 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-105 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-101 | ||||||
回收率：
| The recovery of human CTSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 95 | 87-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.1422.0992.1211.934 101.7651.7981.7821.595 51.2641.2951.2801.093 2.50.7890.8110.8000.613 1.250.6040.6210.6130.426 0.6250.4220.4360.4290.242 0.3120.3020.2990.3010.114 00.1860.1870.187 | |||||||
货期：3-5 working days
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