首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织蛋白酶B (CTSB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTSB含量。CTSB即组织蛋白酶B，是一种溶酶体半胱氨酸蛋白酶。在生理状态下，其参与蛋白降解等正常细胞活动。在病理机制研究里，它与肿瘤进展、神经退行性疾病等多种疾病相关，可能通过促进肿瘤细胞侵袭转移、影响神经细胞功能等方式发挥作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可广泛应用于肿瘤生物学、神经科学及免疫学等基础研究领域，例如评估疾病模型中CTSB表达水平与病理进程的相关性，或用于药物干预实验中对蛋白酶活性调控效果的定量分析，为相关分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amyloid precursor protein secretase ELISA Kit; APP secretase ELISA Kit; APPS ELISA Kit; CATB_HUMAN ELISA Kit; Cathepsin B heavy chain ELISA Kit; Cathepsin B1 ELISA Kit; CathepsinB ELISA Kit; CPSB ELISA Kit; CTSB ELISA Kit; cysteine protease ELISA Kit; OTTHUMP00000116009 ELISA Kit; OTTHUMP00000229510 ELISA Kit; OTTHUMP00000229511 ELISA Kit; OTTHUMP00000229512 ELISA Kit; OTTHUMP00000229514 ELISA Kit; OTTHUMP00000229515 ELISA Kit; OTTHUMP00000229516 ELISA Kit; Preprocathepsin B ELISA Kit
缩写：CTSB
Uniprot No.：P07858
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTSB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 86-92 | |||||
回收率：
| The recovery of human CTSB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 87 | 83-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5442.4132.4792.364 102.0531.9411.9971.882 51.3541.3121.3331.218 2.50.7720.7640.7680.653 1.250.4540.4410.4480.333 0.6250.2820.2970.2900.175 0.3120.1820.1870.1850.070 00.1140.1150.115 | ||||||
货期：3-5 working days
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