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Human neuropeptide Y (NPY) ELISA kit

  • 中文名： 人神经肽Y(NPY) ELISA kit
货号: IPD-E08168h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人神经肽Y(NPY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的NPY含量。NPY即神经肽Y，是一种广泛分布于中枢和外周神经系统的神经肽。它在调节食欲、能量代谢、心血管功能等方面发挥重要作用。研究机制主要围绕其与受体结合，激活下游信号通路来实现生理调节，目前是肥胖、心血管疾病等领域的热门研究靶点。试剂盒检测范围为0.78 pg/mL-50 pg/mL，本产品适用于神经生物学研究、代谢相关疾病机制探索、精神压力模型构建以及药物干预效果评估等科研领域，能够帮助研究人员在体外实验中定量分析NPY的表达动态变化，为神经内分泌系统研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C-flanking peptide of NPY ELISA Kit; CPON ELISA Kit; Neuropeptide tyrosine ELISA Kit; Neuropeptide Y precursor ELISA Kit; NPY ELISA Kit; NPY_HUMAN ELISA Kit; Pro neuropeptide Y ELISA Kit; PYY 4 ELISA Kit; PYY4 ELISA Kit; Y Neuropeptide ELISA Kit
  • 缩写：
    NPY
  • Uniprot No.：
    P01303
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    0.78 pg/mL-50 pg/mL
  • 灵敏度：
    0.195 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:5Average %95
    Range %82-101
    1:10Average %94
    Range %86-103
    1:20Average %87
    Range %82-94
    1:40Average %95
    Range %88-100
  • 回收率：
    The recovery of human NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9589-100
    EDTA plasma (n=4)9085-96














    标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



























































































    pg/mlOD1OD2AverageCorrected
    502.6532.5612.6072.458
    252.1752.2842.2302.081
    12.51.5961.5741.5851.436
    6.250.8950.8630.8790.730
    3.130.5170.5420.5300.381
    1.570.3410.3260.3340.185
    0.780.2780.2660.2720.123
    00.1470.1500.149








  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NPY antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled NPY antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

  • 本试剂盒不含材料：


    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

    • An incubator can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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