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产品描述：人去乙酰化酶Sirtuin-1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SIRT1含量。SIRT1 是一种与多种生理过程相关的靶点。它参与细胞代谢、衰老、应激反应等调节。研究机制上，其通过对蛋白质去乙酰化作用来调控基因表达和细胞功能。在代谢疾病、神经退行性疾病等领域是研究热点，有望为相关疾病治疗提供新策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品适用于基础科研中探索SIRT1在衰老机制、代谢调控或疾病模型中的表达变化，例如评估药物干预、基因编辑或环境因素对细胞/组织内SIRT1活性的影响，也可用于体外培养细胞分泌SIRT1的动力学研究，为分子机制探索和药物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：75SirT1 ELISA Kit; hSIR2 ELISA Kit; hSIRT1 ELISA Kit; HST2 ELISA Kit; HST2, S. cerevisiae, homolog of ELISA Kit; NAD dependent deacetylase sirtuin 1 ELISA Kit; NAD dependent protein deacetylase sirtuin 1 ELISA Kit; NAD-dependent deacetylase sirtuin-1 ELISA Kit; OTTHUMP00000198111 ELISA Kit; OTTHUMP00000198112 ELISA Kit; Regulatory protein SIR2 homolog 1 ELISA Kit; SIR1_HUMAN ELISA Kit; SIR2 ELISA Kit; SIR2 like 1 ELISA Kit; SIR2 like protein 1 ELISA Kit; SIR2, S.cerevisiae, homolog-like 1 ELISA Kit; SIR2-like protein 1 ELISA Kit; SIR2ALPHA ELISA Kit; SIR2L1 ELISA Kit; Sirt1 ELISA Kit; SirtT1 75 kDa fragment ELISA Kit; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ELISA Kit; Sirtuin 1 ELISA Kit; Sirtuin type 1 ELISA Kit
缩写：SIRT1
Uniprot No.：Q96EB6
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-94 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-97 | ||||
| EDTA plasma (n=4) | 96 | 93-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3222.3352.3292.18751.7761.8501.8131.6712.51.2781.2981.2881.1461.250.9290.9180.9240.7820.6250.6400.6230.6320.4900.3120.4490.4400.4450.3030.1560.3030.2880.2960.15400.1440.1400.142 | ||||||
货期：3-5 working days
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