| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FP497; MGC15319; MGC42355; BID |
| Entrez GeneID | 637 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Bid |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Bid抗体的3篇参考文献，按研究内容和应用方向简要总结：
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1. **文献名称**：*Bid, a Bcl-2 interacting protein, mediates cytochrome c release in response to activation of cell surface receptors*
**作者**：Luo, X., Budihardjo, I., Zou, H., et al.
**摘要**：该研究利用Bid特异性抗体，证实了Bid蛋白在死亡受体(如Fas)介导的凋亡信号通路中的作用，揭示了其被caspase-8切割后生成活性片段tBid，进而触发线粒体细胞色素c释放的机制。
2. **文献名称**：*Mitochondrial apoptosis: killing cancer cells by Bid-induced release of cytochrome c*
**作者**：Gross, A., Yin, X.M.
**摘要**：综述中讨论了Bid在连接死亡受体通路与线粒体凋亡通路中的核心作用，强调通过Bid抗体检测其在肿瘤细胞中的激活状态，为靶向Bid的癌症治疗策略提供理论依据。
3. **文献名称**：*BH3 domain of BID is required for interaction with BCL-2 and BCL-XL*
**作者**：Zha, J., Weiler, S., Oh, K.J., et al.
**摘要**：研究通过免疫共沉淀(使用Bid抗体)和突变分析，阐明了Bid蛋白的BH3结构域与抗凋亡蛋白BCL-2/BCL-XL的相互作用机制，为设计BH3类似物类药物奠定基础。
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**备注**：以上文献均涉及Bid蛋白功能及其在凋亡中的机制研究，Bid抗体在实验中主要用于蛋白检测、定位及相互作用验证。如需具体实验细节(如抗体货号、应用场景)，建议进一步查阅原文方法部分。
Bid (BH3-interacting domain death agonist) is a pro-apoptotic protein belonging to the Bcl-2 family, primarily involved in mitochondrial-mediated apoptosis. It functions as a molecular bridge connecting extrinsic (death receptor) and intrinsic (mitochondrial) apoptotic pathways. Upon activation by caspase-8 cleavage during extrinsic apoptosis, truncated Bid (tBid) translocates to mitochondria, where it interacts with other Bcl-2 family proteins to induce mitochondrial outer membrane permeabilization (MOMP), releasing cytochrome c and activating downstream caspases.
Bid antibodies are critical tools for detecting Bid's expression, activation, and subcellular localization in apoptosis research. They enable the identification of full-length Bid (∼22 kDa) and its active fragment tBid (∼15 kDa) via Western blotting, immunohistochemistry, or immunofluorescence. These antibodies are widely used to study Bid's role in physiological processes (e.g., tissue homeostasis) and diseases, including cancer (where Bid dysregulation affects chemoresistance), liver injury, and neurodegenerative disorders.
Research on Bid also explores its non-apoptotic roles, such as regulating DNA repair and cell cycle progression. Bid-deficient mouse models have further elucidated its tissue-specific functions. The development of phospho-specific Bid antibodies has advanced understanding of post-translational modifications influencing its activity. As a key node in apoptotic signaling, Bid remains a focal point for therapeutic strategies targeting cell death pathways.
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