首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人去唾液酸糖蛋白受体(ASGPR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ASGPR含量。ASGPR是一种重要的靶点。它存在于肝脏细胞表面，可识别和结合含半乳糖残基的糖蛋白。研究机制上，利用其特异性结合特性，开发与之结合的药物，实现靶向肝脏递送治疗药物，提高药物疗效，减少对其他组织的副作用。试剂盒检测范围为31.2 ng/mL-2000 ng/mL，适用于基础科研中探索肝脏疾病发生机制、糖蛋白代谢调控研究、药物靶点验证等方向，尤其适合检测肝组织样本中ASGPR的表达动态或循环系统中相关蛋白的浓度变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ASGP-R 1 ELISA Kit; ASGPR 1 ELISA Kit; ASGPR ELISA Kit; ASGPR1 ELISA Kit; Asgr1 ELISA Kit; ASGR1_HUMAN ELISA Kit; Asialoglycoprotein receptor 1 ELISA Kit; C type lectin domain family 4 member H1 ELISA Kit; C-type lectin domain family 4 member H1 ELISA Kit; CLEC4H1 ELISA Kit; Hepatic lectin H1 ELISA Kit; HL-1 ELISA Kit
缩写：ASGPR
Uniprot No.：P07306
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.2 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ASGPR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 83-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of human ASGPR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.7052.6182.6622.55010001.9872.0402.0141.9025001.3591.3411.3501.2382500.7860.7620.7740.6621250.4720.4580.4650.35362.50.3180.3070.3130.20131.20.1810.1860.1840.07200.1090.1150.112 | ||||||
货期：3-5 working days
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