| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABCG2; ABCP; BCRP; BCRP1; MXR; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD338 |
| Entrez GeneID | 9429 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human BCRP/ABCG2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ABCG2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Specific detection of ABCG2/BCRP expression in leukemia stem cells using a novel antibody"*
**作者**: Zhou S, et al.
**摘要**: 本研究开发了一种高特异性单克隆抗体(5D3)，用于检测ABCG2在急性髓系白血病干细胞中的表达，验证了其在流式细胞术和免疫组化中的应用，并证实ABCG2高表达与化疗耐药相关。
2. **文献名称**: *"Comparative analysis of ABCG2 antibodies for immunohistochemical detection in formalin-fixed tissues"*
**作者**: Maliepaard M, et al.
**摘要**: 对比了三种商业ABCG2抗体(如BXP-21、BXP-34)在福尔马林固定组织中的性能，发现BXP-34抗体在乳腺癌和肠癌中染色特异性最佳，强调抗体选择对临床样本分析的重要性。
3. **文献名称**: *"ABCG2-mediated drug resistance: Functional studies using monoclonal antibodies"*
**作者**: Robey RW, et al.
**摘要**: 利用ABCG2特异性抗体(如5D3)通过抑制其转运功能，逆转肿瘤细胞对拓扑替康等药物的耐药性，证实ABCG2在化疗药物外排中的直接作用。
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以上研究涵盖了ABCG2抗体的开发验证、检测优化及功能机制探索，适用于癌症耐药研究和诊断应用。
ABCG2. also known as breast cancer resistance protein (BCRP), is a member of the ATP-binding cassette (ABC) transporter superfamily. It plays a critical role in cellular detoxification and multidrug resistance by actively exporting a wide range of endogenous and exogenous substrates, including chemotherapeutic agents, toxins, and xenobiotics. Expressed in various tissues—such as the placenta, blood-brain barrier, liver, and intestine—ABCG2 contributes to tissue protection and drug pharmacokinetics. Its overexpression in cancer cells is linked to reduced drug accumulation and treatment failure.
ABCG2 antibodies are essential tools for studying the protein’s expression, localization, and function. These antibodies enable detection via techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry, aiding research in oncology (e.g., drug resistance mechanisms), stem cell biology (identifying side population stem cells), and toxicology. Monoclonal antibodies offer high specificity for targeted epitopes, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity but requiring validation to minimize cross-reactivity.
Recent applications include exploring ABCG2’s role in cancer stem cells and developing inhibitors to overcome chemoresistance. Reliable ABCG2 antibodies are crucial for advancing both basic research and therapeutic strategies targeting this transporter.
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