首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人强啡肽(Dyn)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Dyn含量。人强啡肽（Dyn）是一种具有阿片样活性的内源性肽，主要作用于κ-阿片受体，具有镇痛、减轻脑水肿、改善缺血后脑记忆障碍等脑保护作用。其作用机制主要通过阿片样受体途径和非阿片样受体途径实现，如κ受体介导的镇痛和NMDA受体途径的脑保护作用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本试剂盒检测范围为3.12 pg/mL至200 pg/mL，可精准识别低丰度靶标，适用于神经生物学、疼痛机制研究或精神疾病动物模型中的动态监测。操作简便，仅需100 μL样本即可完成检测，配套提供标准品、稀释液及终止液等完整组分，适用于实验室规模的高通量筛查或靶向性研究，为探索Dyn在神经信号传导、药物干预效果评估等科研领域提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Dyn
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 84-99 | ||||
| EDTA plasma (n=4) | 100 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5372.5762.5572.4511002.1412.1642.1532.047501.7781.7911.7851.679251.4051.4551.4301.32412.51.0121.0531.0330.9276.250.6040.6240.6140.5083.120.3480.3570.3530.24700.1050.1070.106 | ||||||
货期：3-5 working days
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