首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前病毒整合位点1(Pim1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的PIM1含量。PIM1 是一个重要的靶点。它属于丝氨酸/苏氨酸激酶家族。在细胞增殖、存活和分化等过程中发挥关键作用。研究发现其异常表达与多种肿瘤发生发展密切相关，目前研究聚焦于揭示其信号通路机制，以开发针对性治疗药物。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肿瘤发生机制研究、药物作用效果评估、细胞信号通路分析等科研场景，支持科研人员高效开展体外分子生物学研究，为探索Pim1在疾病模型中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Oncogene PIM 1 ELISA Kit; Oncogene PIM1 ELISA Kit; PIM 1 ELISA Kit; pim 1 kinase 44 kDa isoform ELISA Kit; Pim 1 kinase ELISA Kit; pim 1 oncogene (proviral integration site 1) ELISA Kit; Pim 1 oncogene ELISA Kit; PIM ELISA Kit; PIM1 ELISA Kit; pim1 kinase 44 kDa isoform ELISA Kit; PIM1_HUMAN ELISA Kit; Pim2 ELISA Kit; PIM3 ELISA Kit; Proto oncogene serine/threonine protein kinase Pim 1 ELISA Kit; Proto-oncogene serine/threonine-protein kinase Pim-1 ELISA Kit; Proviral integration site 1 ELISA Kit; Proviral integration site 2 ELISA Kit
缩写：PIM1
Uniprot No.：P11309
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 88 | ||||
| Range % | 83-91 | |||||
| 1:10 | Average % | 102 | ||||
| Range % | 95-105 | |||||
| 1:20 | Average % | 105 | ||||
| Range % | 100-108 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human PIM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-95 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.4122.3242.3682.260101.8741.8561.8651.75751.2011.2171.2091.1012.50.7010.6880.6950.5871.250.4470.4340.4410.3330.6250.3120.3250.3190.2110.3120.2180.2040.2110.10300.1060.1100.108 | ||||||
货期：3-5 working days
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