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Human secretogranin-1 (chromogranin-B)(CHGB/SCG1)ELISA kit

  • 中文名: 人分泌粒蛋白1(嗜铬粒蛋白B)(CHGB/SCG1)ELISA kit
货号: IPD-E17547h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人分泌粒蛋白1(嗜铬粒蛋白B)(CHGB/SCG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的CHGB含量。试剂盒检测范围为1.56 ng/ml- 100 ng/ml,灵敏度为0.39 ng/ml。该产品可为神经内分泌系统功能研究、疾病模型构建、药物作用机制探索等科研场景提供数据支持,例如评估实验条件下CHGB的分泌动态或病理组织中蛋白表达差异本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    CCB peptide ELISA Kit; Cg B ELISA Kit; CgB ELISA Kit; Chgb ELISA Kit; Chromogranin-B ELISA Kit; ChromograninB ELISA Kit; SCG1 ELISA Kit; SCG1_HUMAN ELISA Kit; Secretogranin 1 ELISA Kit; Secretogranin B ELISA Kit; Secretogranin I ELISA Kit; SgI ELISA Kit
  • 缩写:
    CHGB
  • Uniprot No.:
    P05060
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 ng/ml-100 ng/ml
  • 灵敏度:
    0.39 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CHGB/SCG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    85-97


    1:2

    Average %

    96


    Range %

    92-102


    1:4

    Average %

    102


    Range %

    95-109


    1:8

    Average %

    88


    Range %

    86-90


  • 回收率:

    The recovery of human CHGB/SCG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    86-96


    EDTA plasma (n=4)

    98

    94-102


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.364

    2.560

    2.462

    2.317


    50

    1.901

    1.986

    1.944

    1.799


    25

    1.413

    1.522

    1.468

    1.323


    12.5

    0.972

    1.052

    1.012

    0.867


    6.25

    0.625

    0.624

    0.625

    0.480


    3.125

    0.379

    0.359

    0.369

    0.224


    1.56

    0.264

    0.267

    0.266

    0.121


    0

    0.142

    0.147

    0.145




  • 货期:
    3-5 working days

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