| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII |
| Entrez GeneID | 10125 |
| WB Predicted band size | Calculated MW: 90 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Rasgrp1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RASGRP1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*RASGRP1 deficiency in mice promotes immature B cell survival*
**作者**：Hartzell C. et al.
**摘要**：研究利用RASGRP1敲除小鼠模型，通过Western blot和流式细胞术结合特异性RASGRP1抗体，揭示RASGRP1缺失导致未成熟B细胞存活增加，提示其在B细胞发育中的调控作用。
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2. **文献名称**：*RASGRP1 overexpression in T-cell acute lymphoblastic leukemia drives oncogenic signaling*
**作者**：Li S. et al.
**摘要**：通过免疫组化及共聚焦显微术(使用RASGRP1抗体)，发现RASGRP1在T-ALL患者中异常高表达，激活MAPK通路并促进白血病细胞增殖，为靶向治疗提供依据。
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3. **文献名称**：*Autoimmune-associated RASGRP1 variants alter lymphocyte signaling*
**作者**：Mendoza J.L. et al.
**摘要**：结合CRISPR基因编辑和RASGRP1抗体免疫沉淀技术，验证自身免疫疾病相关RASGRP1突变体导致T细胞信号传导异常，可能与类风湿性关节炎发病机制相关。
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以上文献均通过RASGRP1抗体进行蛋白定位、表达分析或功能研究，涵盖免疫调控及疾病机制领域。
The RASGRP1 (RAS guanyl-releasing protein 1) antibody is a tool used to detect and study the RASGRP1 protein, a key signaling molecule in the RAS-MAPK pathway. RASGRP1 belongs to the guanine nucleotide exchange factor (GEF) family and activates RAS proteins by catalyzing the exchange of GDP for GTP. It plays critical roles in immune cell signaling, particularly in T-cell and B-cell development, differentiation, and activation. Dysregulation of RASGRP1 has been implicated in autoimmune disorders, immunodeficiency, and cancers, such as T-cell lymphoma and leukemia.
RASGRP1 antibodies are widely utilized in research to investigate its expression, localization, and function in cellular pathways. These antibodies enable techniques like Western blotting, immunohistochemistry, and flow cytometry, aiding in the identification of RASGRP1 in tissues or cell lines. Studies using RASGRP1 antibodies have revealed its tissue-specific expression patterns and its role in mediating responses to antigens, cytokines, and growth factors.
Notably, RASGRP1 mutations or altered expression have been linked to autoimmune diseases (e.g., lupus) and hematologic malignancies, making it a potential biomarker or therapeutic target. Researchers also explore its interaction with other signaling components, such as PKC and Ras isoforms, to elucidate mechanisms underlying immune disorders and oncogenesis. Overall, RASGRP1 antibodies are essential for advancing understanding of RASGRP1's biological and pathological significance.
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