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产品描述：人肺表面活性蛋白D(SP-D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、支气管肺泡灌洗液、组织匀浆样本中的SFTPD含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，本试剂盒可精准检测生物样本中SP-D的动态变化，为研究呼吸道疾病发病机制、评估药物干预效果或探索生物标志物提供技术支持，尤其适用于肺损伤模型研究、感染性肺炎病理分析以及肺部微环境变化的体外实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：COLEC 7 ELISA Kit; COLEC7 ELISA Kit; Collectin-7 ELISA Kit; Collectin7 ELISA Kit; Lung surfactant protein D ELISA Kit; PSP D ELISA Kit; PSP-D ELISA Kit; PSP-D Surfactant protein D ELISA Kit; PSPD ELISA Kit; Pulmonary surfactant apoprotein ELISA Kit; Pulmonary surfactant associated protein D ELISA Kit; Pulmonary surfactant associated protein PSP-D ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; SFTP 4 ELISA Kit; SFTP4 ELISA Kit; SFTPD ELISA Kit; SFTPD_HUMAN ELISA Kit; SP D ELISA Kit; SP-D ELISA Kit; Surfactant associated protein pulmonary 4 ELISA Kit; Surfactant protein D ELISA Kit; Surfactant pulmonary associated protein D ELISA Kit
缩写：SFTPD
Uniprot No.：P35247
种属：Homo sapiens (Human)
样本类型：serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-108 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.1982.0682.1332.042401.4571.3781.4181.327200.9250.8840.9050.814100.6000.5780.5890.49850.4100.3930.4020.3112.50.2550.2510.2530.1621.250.1360.1340.1350.04400.0920.0900.091 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SP-D antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SP-D antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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