首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人存活素(Surv)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Surv Ab含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于探索该靶点在细胞凋亡调控与肿瘤发生机制中的科研应用。科研人员可将其用于体外培养细胞上清液、实验动物模型血清或临床前研究血浆样本中Survivin蛋白表达水平的动态监测，为肿瘤发生机制解析、药物敏感性评估及治疗方案优化提供数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Surv Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 81-90 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-101 | ||||
| EDTA plasma (n=4) | 87 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.2132.1572.1852.014 201.6121.5121.5621.391 101.1561.1131.1350.964 50.7790.7350.7570.586 2.50.5220.4920.5070.336 1.250.3830.3660.3750.204 0.6250.2670.2830.2750.104 00.1680.1730.171 | ||||||
货期：3-5 working days
×
