首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脆性组氨酸三联体(FHIT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FHIT含量。FHIT是重要靶点，其为抑癌基因，在多种肿瘤中常出现异常。研究机制显示，它可能参与调控细胞周期、诱导细胞凋亡等过程，对肿瘤发生发展起关键作用，深入探究其功能与机制有助于肿瘤诊断及治疗方案开发。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于体外培养细胞模型、动物组织样本及生物体液中的FHIT表达水平分析；为研究FHIT在肿瘤发生机制、基因功能调控及药物干预效果评估等领域提供标准化检测工具；适用于分子病理学、肿瘤分子生物学及转化医学等基础研究场景，为科研人员探索FHIT相关分子通路提供高效精准的检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FHITBis(5'-adenosyl)-triphosphatase ELISA Kit; EC 3.6.1.29 ELISA Kit; AP3A hydrolase ELISA Kit; AP3Aase ELISA Kit; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase ELISA Kit; Dinucleosidetriphosphatase ELISA Kit; Fragile histidine triad protein ELISA Kit
缩写：FHIT
Uniprot No.：P49789
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FHIT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
回收率：
| The recovery of human FHIT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-100 | |||||
| EDTA plasma (n=4) | 101 | 96-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.0751.9161.9961.90410001.5611.5011.5311.4395001.1741.1531.1641.0722500.8830.8430.8630.7711250.5920.5890.5910.49962.50.3570.3720.3650.27331.20.1960.2010.1990.10700.0890.0940.092 | |||||||
货期：3-5 working days
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