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产品描述：人成纤维细胞生长因子9(FGF9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF9含量。FGF9即成纤维细胞生长因子9，是FGF家族成员。它在胚胎发育、组织修复等生理过程中发挥作用。研究机制上，FGF9主要通过与细胞表面的受体结合，激活下游信号通路，参与调控细胞的增殖、分化等，在多种疾病发病机制研究中受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于研究FGF9在胚胎发育、组织再生、肿瘤微环境或纤维化疾病中的表达调控机制，支持基础科研中对不同来源样本的蛋白水平检测需求，例如药物干预实验或基因编辑模型的表型验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF 9 ELISA Kit; FGF-9 ELISA Kit; FGF9 ELISA Kit; FGF9_HUMAN ELISA Kit; Fibroblast growth factor 9 ELISA Kit; GAF (Glia-activafibroblast growth factor 9 (glia-activating factor) ELISA Kit; GAF ELISA Kit; Glia Activating Factor ELISA Kit; Glia-activating factor ELISA Kit; HBFG 9 ELISA Kit; HBFG9 ELISA Kit; HBGF-9 ELISA Kit; Heparin-binding growth factor 9 ELISA Kit; MGC119914 ELISA Kit; MGC119915 ELISA Kit; SYNS3 ELISA Kit
缩写：FGF9
Uniprot No.：P31371
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human FGF9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-103 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.4522.5102.4812.3592001.4841.5691.5271.4051000.8370.8700.8540.732500.5040.4650.4850.363250.3460.3410.3440.22212.50.2570.2460.2520.1306.250.1870.1810.1840.06200.1210.1220.122 | ||||||
货期：3-5 working days
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