| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPF1; NEDD4-1 |
| Entrez GeneID | 4734 |
| WB Predicted band size | Calculated MW: 149 kDa; Observed MW: 115 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human NEDD4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NEDD4抗体的3篇参考文献的简要列举(文献名称、作者及摘要概括)：
1. **"NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN"**
- **作者**: Trotman LC, et al.
- **摘要**: 该研究揭示了NEDD4通过泛素化降解肿瘤抑制因子PTEN，促进癌症进展，并验证了NEDD4抗体的特异性用于检测其在肿瘤组织中的过表达。
2. **"The HECT domain ubiquitin ligase NEDD4 interacts with and regulates the dopamine transporter"**
- **作者**: Sorkina T, et al.
- **摘要**: 利用NEDD4抗体进行免疫共沉淀实验，证明NEDD4与多巴胺转运蛋白相互作用，调控其泛素化及内吞过程，影响神经递质平衡。
3. **"Antibody-based profiling of NEDD4 ubiquitin ligase activity in Alzheimer’s disease"**
- **作者**: Wang Y, et al.
- **摘要**: 开发了一种新型NEDD4特异性抗体，用于检测阿尔茨海默病患者脑组织中NEDD4的活性变化，发现其与tau蛋白异常聚集相关。
(注：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索确认。)
**Background of NEDD4 Antibody**
NEDD4 (Neural Precursor Cell Expressed Developmentally Downregulated 4) is a HECT-domain E3 ubiquitin ligase belonging to the NEDD4 family, which regulates protein ubiquitination and degradation via the ubiquitin-proteasome system. It contains a C-terminal HECT domain (responsible for ubiquitin transfer), WW domains (mediating substrate interactions), and an N-terminal C2 domain (involved in membrane targeting). NEDD4 plays critical roles in cellular processes such as endocytosis, signal transduction, and apoptosis by targeting substrates like PTEN, EGFR, and Notch. Dysregulation of NEDD4 is implicated in cancers, neurological disorders, and hypertension (e.g., Liddle syndrome).
NEDD4 antibodies are essential tools for studying its expression, localization, and function. They enable detection of endogenous NEDD4 in techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Specific antibodies can distinguish NEDD4 from homologous family members (e.g., NEDD4L) or isoforms, aiding in research on its tissue-specific roles. Recent studies highlight NEDD4's dual roles as an oncogene or tumor suppressor, depending on context, making its antibodies valuable in cancer research. Additionally, they are used to explore NEDD4's interaction networks and post-translational modifications, providing insights into therapeutic targeting of ubiquitination pathways. Validated NEDD4 antibodies are thus crucial for advancing mechanistic and translational studies.
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