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Human Interleukin 22(IL-22) ELISA KIT

  • 中文名： 人白介素22(IL-22)ELISA Kit
货号: IPD-E13418h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素22(IL-22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL22含量。IL-22是一种细胞因子，主要由Th17等细胞产生。它通过与受体结合激活下游信号通路。IL-22在黏膜屏障维护、组织修复及炎症反应中发挥重要作用，其异常表达与多种疾病如自身免疫病、感染性疾病发病机制有关，是研究热点。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。科研领域可应用于免疫调节机制研究、炎症模型构建、药物干预效果评估等方向，为探索IL - 22在组织稳态维持、病原体防御及上皮修复中的分子机制提供可靠工具。适用于实验室常规检测及高通量样本分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cytokine Zcyto18 ELISA Kit; IL 10 related T cell derived inducible factor ELISA Kit; IL 21 ELISA Kit; IL 22 ELISA Kit; IL D110 ELISA Kit; IL TIF ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-22 ELISA Kit; IL-TIF ELISA Kit; IL21 ELISA Kit; Il22 ELISA Kit; IL22_HUMAN ELISA Kit; ILD110 ELISA Kit; ILTIF ELISA Kit; Interleukin 10 related T cell derived inducible factor ELISA Kit; interleukin 21 ELISA Kit; Interleukin 22 ELISA Kit; Interleukin-22 ELISA Kit; MGC79382 ELISA Kit; MGC79384 ELISA Kit; TIFa ELISA Kit; TIFIL 23 ELISA Kit; TIFIL23 ELISA Kit; UNQ3099/PRO10096 ELISA Kit; zcyto18 ELISA Kit
  • 缩写：
    IL22
  • Uniprot No.：
    Q9GZX6
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/ml - 5000 pg/ml
  • 灵敏度：
    19.5 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    103


    Range %

    99-109


    1:2

    Average %

    109


    Range %

    105-115


    1:4

    Average %

    98


    Range %

    91-105


    1:8

    Average %

    93


    Range %

    86-100


  • 回收率：

    The recovery of human IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    107

    103-111


    EDTA plasma (n=4)

    99

    92-106


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    5000

    2.994

    3.064

    3.029

    2.921


    2500

    2.338

    2.307

    2.322

    2.215


    1250

    1.400

    1.358

    1.379

    1.272


    625

    0.719

    0.729

    0.724

    0.617


    312

    0.371

    0.391

    0.381

    0.274


    156

    0.271

    0.263

    0.267

    0.160


    78

    0.196

    0.184

    0.190

    0.083


    0

    0.110

    0.105

    0.107



  • 本试剂盒所含材料：


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-22 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled IL-22 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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