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产品描述：人α淀粉酶(AMY1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、唾液、组织匀浆样本中的AMY1含量。试剂盒检测范围为7.8 IU/mL-500 IU/mL，适用于科研领域中对消化酶活性动态的定量分析，如探究饮食习惯对唾液淀粉酶分泌的影响、疾病模型中胰腺外分泌功能评估，或通过组织匀浆分析特定器官的酶活性分布。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit
缩写：AMY1
Uniprot No.：P04745
种属：Homo sapiens (Human)
样本类型：serum, saliva, tissue homogenates
检测范围：7.8 IU/mL-500 IU/mL
灵敏度：1.95 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-93 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 96-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected5002.9912.7892.8902.7882502.6072.6042.6062.5041252.2542.2132.2342.13262.51.6821.5691.6261.52431.20.9310.9240.9280.82615.60.5220.5130.5180.4167.80.2910.2880.2900.18800.1060.0980.102 | ||||||
货期：3-5 working days
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