| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147 |
| Entrez GeneID | 7706 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human TRIM25 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于TRIM25抗体的3篇参考文献，涵盖其功能及实验应用：
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1. **文献名称**：*TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity*
**作者**：Gack, M.U. et al.
**摘要**：该研究揭示了TRIM25通过K63泛素化修饰RIG-I，激活抗病毒信号通路。实验中利用TRIM25抗体进行免疫共沉淀(Co-IP)和Western blot，验证了TRIM25与RIG-I的相互作用，为先天免疫机制提供了关键证据。(*Nature*, 2007)
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2. **文献名称**：*TRIM25 promotes cell proliferation and regulates cellular response to DNA damage through USP15/p53 axis*
**作者**：Zhang, Y. et al.
**摘要**：研究证明TRIM25通过调控USP15-p53通路影响细胞增殖和DNA损伤应答。作者使用TRIM25抗体进行免疫荧光和ChIP实验，证实其在癌细胞中的核定位及靶基因调控作用。(*Cell Reports*, 2019)
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3. **文献名称**：*Ubiquitination of TRIM25 enhances Toll-like receptor signaling in HSV-1 infection*
**作者**：Li, S. et al.
**摘要**：该文献发现TRIM25的泛素化修饰可增强TLR信号通路，促进HSV-1感染后的炎症反应。研究通过TRIM25抗体的免疫印迹(Western blot)和流式细胞术，分析了其在病毒感染中的表达动态。(*Journal of Virology*, 2020)
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这些研究均通过TRIM25抗体进行蛋白质互作、定位或表达分析，展示了其在抗病毒、癌症及炎症中的多功能性。如需更多文献，可进一步限定研究领域或实验方法。
TRIM25 (Tripartite Motif-containing protein 25), also known as E3 ubiquitin-protein ligase TRIM25. is a member of the TRIM family of proteins characterized by a conserved tripartite motif comprising a RING domain, B-box domains, and a coiled-coil region. It plays a critical role in various cellular processes, including innate immunity, antiviral defense, and post-translational protein modification. TRIM25 is best known for its E3 ubiquitin ligase activity, which mediates the addition of ubiquitin molecules to target proteins, influencing their stability, localization, or interactions. Notably, TRIM25 ubiquitinates the N-terminal CARD domain of RIG-I (Retinoic Acid-Inducible Gene I), a key sensor of viral RNA, thereby activating RIG-I-mediated signaling to induce type I interferon production during viral infections. Dysregulation of TRIM25 has been implicated in immune disorders, cancer progression, and neurodegenerative diseases, highlighting its diverse functional roles.
TRIM25 antibodies are essential tools for studying its expression, interactions, and biochemical functions. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). High-quality TRIM25 antibodies exhibit specificity for distinct epitopes, enabling researchers to investigate its subcellular localization, tissue distribution, and post-translational modifications. Commercially available antibodies are often validated in knockout cell lines or tissues to confirm target specificity. Additionally, TRIM25 antibodies contribute to exploring its dual roles in antiviral responses (e.g., during influenza or SARS-CoV-2 infection) and oncogenic or tumor-suppressive activities, depending on cellular context. Understanding TRIM25's regulatory mechanisms through antibody-based assays remains pivotal for dissecting its contributions to disease pathways and therapeutic targeting.
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