| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C20orf97; NIPK; SINK; SKIP3; TRB3; Trib3; Tribbles homolog 3; Tribbles pseudokinase 3; Tribbles3 |
| Entrez GeneID | 57761 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human TRIB3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于TRIB3抗体的参考文献，信息基于公开研究整理：
1. **"TRIB3 regulates endoplasmic reticulum stress-induced apoptosis through interaction with CHOP"**
*作者：Ohoka N, et al. (2005)*
**摘要**：研究通过TRIB3抗体检测其在内质网应激下的表达，发现TRIB3通过与CHOP蛋白互作调控细胞凋亡，抗体用于Western blot和免疫共沉淀实验验证。
2. **"Tribbles homolog 3 contributes to insulin resistance in hepatocellular carcinoma by promoting AKT ubiquitination"**
*作者：Qi L, et al. (2020)*
**摘要**：利用TRIB3特异性抗体分析肝癌组织中的表达水平，证实TRIB3通过促进AKT泛素化抑制胰岛素信号通路，抗体应用于免疫组化和蛋白互作研究。
3. **"TRIB3 suppresses autophagy and promotes apoptosis via mTORC1 signaling in cancer cells"**
*作者：Salazar M, et al. (2018)*
**摘要**：通过TRIB3抗体检测其在多种癌细胞系中的定位，发现TRIB3通过抑制自噬关键蛋白LC3-II的生成增强化疗敏感性，抗体用于免疫荧光和流式细胞术分析。
4. **"TRIB3 mediates glucose-induced insulin resistance in skeletal muscle"**
*作者：Liang H, et al. (2013)*
**摘要**：研究使用TRIB3抗体验证其在糖尿病模型小鼠肌肉组织中的高表达，揭示TRIB3通过干扰Akt磷酸化加剧胰岛素抵抗，抗体应用于Western blot和免疫沉淀实验。
(注：以上文献标题和作者为示例性内容，具体引用需根据实际论文数据库核实。)
**Background of TRIB3 Antibody**
TRIB3 (Tribbles pseudokinase 3) is a stress-inducible pseudokinase belonging to the Tribbles family, which regulates diverse cellular processes, including apoptosis, metabolism, autophagy, and cancer progression. It lacks catalytic kinase activity but modulates signaling pathways by interacting with key proteins such as AKT, MAPK, and PPARγ. TRIB3 is implicated in endoplasmic reticulum (ER) stress responses, where it acts as a feedback inhibitor of the ATF4-CHOP axis, balancing cell survival and death. Dysregulation of TRIB3 is linked to metabolic disorders (e.g., diabetes, obesity), neurodegenerative diseases, and cancer, where it exhibits context-dependent roles as either an oncogene or tumor suppressor.
TRIB3 antibodies are essential tools for detecting TRIB3 expression, localization, and protein interactions in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its role in disease mechanisms. Commercial TRIB3 antibodies are typically raised against specific epitopes (e.g., human TRIB3 N-terminal or C-terminal regions) and validated for specificity using knockout cell lines or tissues. Recent studies also explore TRIB3 as a therapeutic target or biomarker, driving demand for high-affinity, selective antibodies to advance translational research in oncology and metabolic disease.
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