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Human Collagen, type V, alpha 1 (COL5A1)ELISA Kit

  • 中文名： 人V型胶原α1(COL5A1)ELISA Kit
货号: IPD-E13447h
Price： ￥2500
数量：
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  • 产品描述：人V型胶原α1(COL5A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL5A1含量。COL5A1 是一种参与构成细胞外基质的基因，在维持组织的结构和功能方面起关键作用。研究发现其表达异常与多种疾病如癌症、纤维化等相关，机制可能涉及调控细胞增殖、迁移和侵袭，以及影响肿瘤微环境，有望成为疾病治疗潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域对胶原代谢相关机制的研究，例如探究纤维化疾病模型中的基质重塑、皮肤组织工程中的胶原合成动态，或心血管疾病中血管壁结构异常的分子机制。其兼容多种生物样本的特性，为体外实验提供灵活选择，支持基础研究中的蛋白表达水平分析与病理生理学机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：AB collagen ELISA Kit; Alpha 1 type V collagen ELISA Kit; Alpha 2 type V collagen ELISA Kit; CO5A1_HUMAN ELISA Kit; COL5A1 ELISA Kit; Col5A2 ELISA Kit; COL5A2 protein ELISA Kit; Col5A3 ELISA Kit; Collagen alpha 1(V) chain ELISA Kit; Collagen alpha 2 (V) chain precursor ELISA Kit; Collagen alpha 2(V) chain ELISA Kit; Collagen alpha 3(V) chain ELISA Kit; Collagen alpha-1(V) chain ELISA Kit; Collagen fetal membrane A polypeptide ELISA Kit; Collagen type V alpha 1 ELISA Kit; Collagen type V alpha 2 ELISA Kit; Collagen type V alpha 3 ELISA Kit; Collagen V alpha 2 polypeptide ELISA Kit; CollagenV ELISA Kit; MGC105115 ELISA Kit; OTTHUMP00000064637 ELISA Kit; Pro alpha 1 type V collagen ELISA Kit; Pro alpha 3(V) collagen ELISA Kit; Procollagen alpha 2(V) ELISA Kit; Type V preprocollagen alpha 2 chain ELISA Kit

  • 缩写：COL5A1

  • Uniprot No.：P20908

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.312 ng/mL-20 ng/mL

  • 灵敏度：0.078 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human COL5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



    		SampleSerum(n=4)
    1:1Average %94
    Range %85-97

    1:2Average %102
    Range %95-106

    1:4Average %88
    Range %84-92

    1:8Average %91
    Range %84-95

  • 回收率：

    The recovery of human COL5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9792-101
    EDTA plasma (n=4)9387-97














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected 202.3312.2432.2872.122 101.3681.4671.4181.253 50.8570.7960.8270.662 2.50.5080.5270.5180.353 1.250.3840.3720.3780.213 0.6250.2680.2770.2730.108 0.3120.2180.2270.2230.058 00.1640.1650.165  






  • 货期：3-5 working days

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