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产品描述：人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的HELIXⅡ含量。试剂盒检测范围为1.25 ng/mL-80 ng/mL，Ⅱ型胶原是关节软骨的主要结构蛋白，其螺旋结构域（HELIX - Ⅱ）的降解产物可作为软骨代谢异常的分子标志物，在骨关节疾病机制研究、软骨损伤动态监测及药物干预效果评价等领域具有重要科研价值。研究人员可通过该产品探究骨关节炎、类风湿性关节炎等疾病进程中软骨基质降解的动态变化，或用于筛选调节软骨代谢的潜在化合物，为相关领域的分子机制研究和治疗策略开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
缩写：HELIXⅡ
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-112 | |||||
回收率：
| The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 91 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 802.1782.1142.1462.043 401.6741.5811.6281.525 201.1161.0611.0890.986 100.6260.6030.6150.512 50.4230.4070.4150.312 2.50.2520.2390.2460.143 1.250.1930.1840.1890.086 00.1040.1010.103 | ||||||
货期：3-5 working days
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