关键信息
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基因名
PTPRC/CD45R0
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简介
Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. PTPRC/CD45R0 Protein, Human (HEK293, Fc) is the recombinant human-derived PTPRC/CD45R0 protein, expressed by HEK293 , with C-hFc labeled tag.
- 应用
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别名
Receptor-type tyrosine-protein phosphatase C; L-CA; T200; PTPRC; CD45
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P08575-4
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表达区间
Q26-K416
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氨基酸序列
MTMYLWLKLLAFGFAFLDTEVFVTGQSPTPSPTDAYLNASETTTLSPSGSAviSTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
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蛋白长度
Partial
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分子量
122 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PTPRC, also known as CD45, is a crucial receptor-type protein tyrosine phosphatase that plays a vital role in regulating immune system functions, particularly in T and B lymphocytes. The CD45R0 isoform, in particular, is expressed on memory T cells and plays a significant role in their activation and differentiation. Understanding the structural and functional characteristics of PTPRC/CD45R0 is essential for elucidating its mechanisms in adaptive immunity and potential therapeutic applications. Research has shown that dysregulation of CD45 is associated with various autoimmune diseases, cancers, and immune deficiencies, making it a critical target for therapeutic intervention. Recent advances in recombinant protein technology have enabled the production of functional CD45R0 proteins, providing invaluable tools for studying its role in immune signaling pathways and cell interactions. These recombinant proteins can be utilized for antibody production, receptor signaling studies, and drug development, contributing to our understanding of immune responses and the development of novel therapies for immune-related disorders. The exploration of PTPRC/CD45R0 at the molecular level, therefore, holds promise for advancing our knowledge in immunology and enhancing clinical approaches to treat immune-mediated diseases.
