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产品描述：人Ⅰ型胶原α1(COL1A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的COL1A1含量。COL1A1编码Ⅰ型胶原的α1链，在细胞外基质形成中起重要作用。其异常表达与多种疾病相关，如纤维化疾病和肿瘤。研究机制主要聚焦于其在疾病发生发展中对细胞增殖、迁移、侵袭等的影响及相关信号通路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外细胞培养模型中胶原代谢研究、纤维化疾病机制探索或组织工程材料中细胞基质成分分析等科研场景，为研究胶原相关生理病理过程提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 type I collagen ELISA Kit; Alpha 2 type I collagen ELISA Kit; alpha 2 type I procollagen ELISA Kit; alpha 2(I) procollagen ELISA Kit; alpha 2(I)-collagen ELISA Kit; Alpha-1 type I collagen ELISA Kit; alpha1(I) procollagen ELISA Kit; CO1A1_HUMAN ELISA Kit; COL1A1 ELISA Kit; COL1A2 ELISA Kit; collagen alpha 1 chain type I ELISA Kit; Collagen alpha-1(I) chain ELISA Kit; collagen alpha-1(I) chain preproprotein ELISA Kit; Collagen I alpha 1 polypeptide ELISA Kit; Collagen I alpha 2 polypeptide ELISA Kit; collagen of skin; tendon and bone; alpha-1 chain ELISA Kit; collagen of skin; tendon and bone; alpha-2 chain ELISA Kit; Collagen type I alpha 1 ELISA Kit; Collagen type I alpha 2 ELISA Kit; EDSC ELISA Kit; OI1 ELISA Kit; OI2 ELISA Kit; OI3 ELISA Kit; OI4 ELISA Kit; pro-alpha-1 collagen type 1 ELISA Kit; type I proalpha 1 ELISA Kit; type I procollagen alpha 1 chain ELISA Kit; Type I procollagen ELISA Kit
缩写：COL1A1
Uniprot No.：P02452
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-101 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of human COL1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-98 | ||||
| EDTA plasma (n=4) | 99 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.5432.5602.5522.364202.0322.1032.0681.880101.5541.6001.5771.38951.1731.1861.1800.9922.50.6180.6700.6440.4561.250.4250.4300.4280.2400.6250.2980.3100.3040.11600.1810.1950.188 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human COL1A1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled COL1A1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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