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产品描述：犬血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ANG-Ⅱ含量。ANG - Ⅱ即血管紧张素Ⅱ，是肾素 - 血管紧张素系统的关键效应肽。它在血压调节、水电解质平衡等方面发挥重要作用。研究机制上，主要通过与特异性受体结合，激活一系列细胞内信号通路，引发血管收缩、醛固酮释放等生理反应。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研场景下评估药物干预、疾病模型或生理状态对ANG - Ⅱ水平的影响，例如犬类高血压机制探索、肾脏疾病相关生物标志物分析或激素调控研究，为兽医科研及动物模型开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：angiotension Ⅱ, angiotensin 2, ANG2
缩写：ANG-Ⅱ
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 86-93 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of dog ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 100 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4001.9782.0302.0041.8442001.3241.3951.3601.2001000.7640.8100.7870.627500.4660.5010.4840.324250.3010.3100.3060.14612.50.2540.2700.2620.1026.250.2040.2110.2080.04800.1580.1620.160 | ||||||
货期：3-5 working days
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