首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰多肽酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PPY含量。PPY即多肽YY，是由肠道L细胞分泌的一种激素。它能调节食欲、能量代谢等。研究机制上，其可与特定受体结合，作用于下丘脑等脑区以抑制食欲；也能影响胃肠道运动和消化液分泌，在维持机体能量稳态等生理过程中发挥重要作用。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于科研场景中探究胰多肽在消化系统疾病模型、代谢调控机制或药物干预实验中的动态变化，为动物实验提供可靠的内分泌指标检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PPY
Uniprot No.：P06303
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 104 | |||||
| Range % | 93-109 | ||||||
| 1:200 | Average % | 100 | |||||
| Range % | 91-104 | ||||||
| 1:400 | Average % | 97 | |||||
| Range % | 88-101 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 82-94 | ||||||
回收率：
| The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 83-94 | |||||
| EDTA plasma (n=4) | 100 | 91-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 502.0691.9322.0011.899 251.4291.4661.4481.346 12.50.9460.9170.9320.830 6.250.6620.6510.6570.555 3.120.3480.3400.3440.242 1.560.2420.2370.2400.138 0.780.1640.1590.1620.060 00.1040.1000.102 | |||||||
货期：3-5 working days
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