首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脱氧胶原吡啶交联(DPD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DPD含量。DPD即二氢嘧啶脱氢酶。它是氟尿嘧啶等嘧啶类药物代谢关键酶。其活性水平影响药物疗效与毒性。研究机制主要聚焦于其基因多态性对活性的影响，以及通过调控DPD活性来优化嘧啶类药物治疗方案、降低不良反应。试剂盒检测范围为1.56 pmol/L-100 pmol/L，适用于骨代谢动物模型研究、抗骨质疏松药物疗效评价、骨关节炎病理机制探索等科研领域，支持多种生物样本类型检测，重复性良好且无需复杂前处理，为骨吸收相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DPD
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pmol/L-100 pmol/L
灵敏度：0.39 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-100 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 87-93 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of rat DPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 98 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/L.OD1OD2AverageCorrected1002.4632.5812.5222.391502.1132.1532.1332.002251.5831.6231.6031.47212.50.9420.9510.9470.8166.250.5120.5210.5170.3863.120.3180.3270.3230.1921.560.2220.2360.2290.09800.1300.1320.131 | ||||||
货期：3-5 working days
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