关键信息
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基因名
NHEJ1
- 应用
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别名
XLF; Cernunnos; XRCC4-like factor
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H9Q4
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表达区间
Met1~Ser299
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分子量
43kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NHEJ1, also known as non-homologous end joining factor 1, plays a crucial role in the DNA repair process, specifically in the non-homologous end joining (NHEJ) pathway. This pathway is essential for repairing double-strand breaks (DSBs) in DNA, which can arise from various sources, including ionizing radiation, reactive oxygen species, and during cellular processes like V(D)J recombination. Dysfunctions in the NHEJ pathway, due to mutations in key components like NHEJ1, can lead to genomic instability, cancer, and immunodeficiency disorders. Recent research has highlighted the importance of NHEJ1 in maintaining genomic integrity by aiding in the recruitment of other repair proteins to sites of DNA damage. Understanding the structure and functional mechanisms of NHEJ1 could provide insights into its interactions with other proteins involved in DSB repair and may unveil potential therapeutic targets for enhancing DNA repair processes in cancer treatment or addressing repair deficiencies in various diseases. Studies employing recombinant NHEJ1 proteins have advanced our understanding of its biochemical properties, interactions, and influence on cellular responses to DNA damage, making it a focal point in the broader field of DNA repair research.
