首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人纤维蛋白原（Fb）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Fb含量。试剂盒检测范围为0.312 μg/mL-20 μg/mL，该产品适用于基础医学研究中凝血/纤溶系统调控机制探索、肝脏功能评估、心血管疾病动物模型验证，以及炎症相关病理过程的分子机制研究，为体外药物筛选、疾病模型构建和生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Fb
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 98 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected 202.6482.7012.6752.583 102.1592.1692.1642.072 51.4761.5361.5061.414 2.50.8640.8760.8700.778 1.250.4430.4750.4590.367 0.6250.2340.2530.2440.152 0.3120.1460.1540.1500.058 00.0950.0890.092 | ||||||
货期：3-5 working days
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