首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的Col Ⅳ含量。Col Ⅳ（IV型胶原蛋白）是细胞基底膜的主要结构成分，参与组织完整性维持和正常功能。其研究机制涉及细胞外基质组成、细胞黏附、迁移、生存、扩张和分化。在疾病如肿瘤、肝纤维化等中，Col Ⅳ的表达和功能异常，成为治疗研究的重要靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本产品适用于体外研究基底膜相关疾病机制、评估药物对细胞外基质代谢的影响，或通过体液样本分析Col Ⅳ作为生物标志物的潜在价值，为组织修复、血管生成及纤维化研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
缩写：Col Ⅳ
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 86 | ||||
| Range % | 80-104 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 92-113 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 93 | 85-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.7482.8562.8022.635 20002.4592.4362.4482.281 10001.8761.9681.9221.755 5001.2401.2341.2371.070 2500.8430.8360.8400.673 1250.4340.4470.4410.274 62.50.2460.2560.2510.084 00.1650.1680.167 | ||||||
货期：3-5 working days
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