首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脱氧吡啶酚/脱氧吡啶啉(DPD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的DPD含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可满足骨代谢疾病机制研究、骨质疏松模型评估、骨折愈合监测或肿瘤骨转移实验等科研需求；适用于实验室常规骨代谢相关生物标志物的定量检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：DPD
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-106 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 80-98 | |||||
回收率：
| The recovery of human DPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.1162.0932.1051.955501.9121.8031.8581.708251.6631.5741.6191.46912.51.2581.1861.2221.0726.250.8940.8670.8810.7313.120.5210.5020.5120.3621.560.3140.2890.3020.15200.1520.1480.150 | ||||||
货期：3-5 working days
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