首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人同型半胱氨酸(Hcy)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代谢中间产物。其水平异常升高被视为心脑血管疾病等的独立危险因素。它会损伤血管内皮细胞，促进血栓形成，影响脂质代谢，还能引发氧化应激反应，干扰正常细胞功能。试剂盒检测范围为0.78 nmol/mL-50 nmol/mL，本产品适用于疾病分子机制研究、营养代谢分析、药物干预效果评估及生物标志物筛查等科研场景，为实验室提供标准化、高重复性的Hcy检测方案，仅限科学研究使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Hcy
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：0.78 nmol/mL-50 nmol/mL
灵敏度：0.195 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-101 | ||||
| EDTA plasma (n=4) | 92 | 89-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2AverageCorrected502.0822.1562.1191.997251.4561.4891.4731.35112.50.9410.9680.9550.8336.250.5830.5970.5900.4683.1250.3420.3670.3550.2331.560.2480.2610.2550.1330.780.1920.1980.1950.07300.1210.1230.122 | ||||||
货期：3-5 working days
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