首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗甲状腺球蛋白抗体(ATGA/TGAB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的TG Ab含量。TG Ab即甲状腺球蛋白抗体，是自身免疫性甲状腺疾病患者血清中常见的自身抗体。其背景与自身免疫紊乱相关。研究机制上，它可能通过破坏甲状腺滤泡细胞、影响甲状腺球蛋白代谢等，参与自身免疫性甲状腺炎的发病过程，影响甲状腺功能。试剂盒检测范围为1.25 U/mL-20 U/mL，适用于血清、血浆样本的科研分析；在动物模型中常被用于研究甲状腺自身免疫反应的病理机制及药物干预效果评估；适用于实验室环境下的小鼠模型研究，包括甲状腺自身免疫疾病发病机理探索、免疫调节药物作用效果验证以及相关生物标志物动态监测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TG Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：1.25 U/mL-20 U/mL
灵敏度：1.25 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-101 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-98 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 84-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-103 | |||||
| EDTA plasma (n=4) | 95 | 90-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2Average 200.1460.1510.149 100.2780.2810.280 50.4570.4630.460 2.50.8540.8700.862 1.251.8651.8281.847 | |||||||
货期：3-5 working days
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