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Rabbit Monoclonal RENT1 Antibody

  • 中文名： RENT1抗体
  • 别    名： UPF1; KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1
货号: IPDX21945
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUPF1; KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1
Entrez GeneID5976
WB Predicted band sizeCalculated MW: 124 kDa; Observed MW: 124 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human RENT1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于RENT1抗体的参考文献示例(注：RENT1即UPF2.参与无义介导的mRNA降解途径)：

1. **"Characterization of Rent1. a trans-regulator of nonsense-mediated mRNA decay in mammalian cells"**

- **作者**: Serin G, et al. (2001)

- **摘要**: 研究鉴定了RENT1(UPF2)作为哺乳动物NMD途径的核心组分，利用特异性抗体证实其与UPF1的相互作用，并揭示其在mRNA质量控制中的作用。

2. **"Rent1/Upf2 is essential for embryonic viability and regulation of nonsense transcripts"**

- **作者**: Mendell JT, et al. (2003)

- **摘要**: 通过基因敲除小鼠模型，证明RENT1对胚胎发育至关重要，使用抗体检测组织中的蛋白表达缺失，并发现其突变导致异常mRNA积累。

3. **"The role of Upf proteins in modulating the translation read-through of nonsense-containing transcripts"**

- **作者**: Kashima I, et al. (2006)

- **摘要**: 利用RENT1抗体进行免疫共沉淀实验，阐明UPF1-UPF2复合物在识别提前终止密码子及触发mRNA降解中的分子机制。

4. **"Nonsense-mediated mRNA decay: from mechanistic understanding to clinical applications"**

- **作者**: Brogna S, et al. (2009)

- **摘要**: 综述中总结了RENT1抗体的应用，包括其在NMD通路蛋白互作研究和疾病(如遗传性癌症)中的异常表达检测。

(注：以上文献信息为示例，实际引用需核对原文准确性。)

背景信息

The RENT1 antibody targets the RENT1 (Regulator of Nonsense Transcripts 1) protein, also known as UPF2 (Up-Frameshift Protein 2), a critical component of the nonsense-mediated mRNA decay (NMD) pathway. NMD is a conserved surveillance mechanism that detects and degrades mRNAs containing premature termination codons (PTCs), preventing the production of truncated, potentially toxic proteins. RENT1/UPF2 functions as a scaffold, bridging interactions between UPF3 and UPF1 to form the NMD surveillance complex, which identifies PTCs during translation. Dysregulation of RENT1 is linked to various diseases, including cancers, neurodegenerative disorders, and genetic conditions caused by nonsense mutations. The RENT1 antibody is widely used in research to study NMD mechanisms, protein expression levels, and subcellular localization. It serves as a tool for investigating how NMD contributes to cellular homeostasis, disease pathogenesis, and therapeutic strategies targeting mRNA stability. Studies utilizing this antibody have provided insights into the role of RENT1 in cancer progression, viral infection responses, and the regulation of stress granules. Its application spans techniques like Western blotting, immunofluorescence, and co-immunoprecipitation, aiding in the exploration of RNA quality control pathways and their implications in precision medicine.

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