首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗Smith抗体(Sm)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Sm Ab含量。Sm Ab即抗史密斯抗体，是系统性红斑狼疮（SLE）的血清标志性抗体。其背景在于SLE是一种自身免疫病。研究机制方面，它能识别细胞核中的特定蛋白，可作为SLE诊断的重要依据，在自身免疫反应和疾病发生发展机制研究中有重要意义。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对系统性红斑狼疮（SLE）相关模型的抗体水平分析；可广泛应用于小鼠疾病模型中自身抗体动态监测、药物疗效评估或免疫病理学研究；为探索SLE发病机理及潜在治疗靶点提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Sm Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.8842.7522.8182.614 202.3452.2762.3112.107 101.5521.4951.5241.320 50.8360.8010.8190.615 2.50.5270.5240.5260.322 1.250.3210.3440.3330.129 0.6250.2550.2590.2570.053 00.2010.2060.204 | ||||||
货期：3-5 working days
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