首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗平滑肌抗体(ASMA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ASM Ab含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品可为研究者提供小鼠模型中ASMA水平的动态监测，支持自身免疫性疾病机制探索、药物干预效果评估及病理模型构建等科研需求，尤其适用于肝脏、血管相关疾病动物实验的样本分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ASM Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ASMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 87-102 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 84-93 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 98-111 | ||||||
回收率：
| The recovery of mouse ASMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 81-94 | |||||
| EDTA plasma (n=4) | 114 | 102-117 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1002.5612.4982.5302.397501.4131.4561.4351.302250.6950.7020.6990.56612.50.4090.4170.4130.2806.250.2920.3040.2980.1653.120.1900.1980.1940.0611.560.1660.1710.1690.03600.1360.1300.133 | |||||||
货期：3-5 working days
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