| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pellino-related intracellular-signaling molecule; RING-type E3 ubiquitin transferase pellino homolog 1 |
| Entrez GeneID | 57162 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of human Pellino 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Pellino 1抗体的参考文献，涵盖其功能及实验应用：
1. **"Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 1 (IRAK-1)-IRAK-4 complex"**
*作者：Muroi, M., & Tanamoto, K. (2008)*
摘要：该研究通过免疫共沉淀和Western blot技术，利用Pellino 1抗体证明其与IRAK-1/IRAK-4复合物的相互作用，阐明了Pellino 1在IL-1信号通路中调控NF-κB激活的关键作用。
2. **"The E3 ligase Pellino 1 promotes innate immune signaling by regulating the stability of the adaptor Mal"**
*作者：Jiang, Z., et al. (2013)*
摘要：研究通过RNA干扰和Pellino 1抗体介导的Western blot分析，发现Pellino 1通过泛素化修饰适配蛋白Mal，增强TLR2/4信号传导，揭示了其在先天免疫应答中的新机制。
3. **"Pellino 1 regulates the intensity of T cell activation via ubiquitination of HPK1"**
*作者：Yang, C., et al. (2020)*
摘要：利用Pellino 1抗体进行免疫荧光和流式细胞术，研究显示Pellino 1通过调控HPK1的泛素化影响T细胞受体信号强度，为自身免疫疾病治疗提供了潜在靶点。
4. **"Structural basis for Pellino 1-mediated ubiquitination in IRAK signaling"**
*作者：Butchar, J. P., et al. (2017)*
摘要：结合X射线晶体学及Pellino 1抗体的功能实验，解析了Pellino 1的RING结构域如何催化IRAK1泛素化，阐明其在炎症反应中的分子机制。
以上文献均明确使用了Pellino 1抗体进行蛋白互作、定位或功能验证实验，涵盖免疫、炎症及细胞信号领域。
The Pellino family of proteins, including Pellino 1 (PELI1), are evolutionarily conserved E3 ubiquitin ligases critical in regulating innate immune signaling pathways. Pellino 1 was initially identified as an interactor of the serine/threonine kinase IRAK1 in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling cascades. It functions by mediating K63-linked polyubiquitination of key signaling molecules, such as IRAK1 and TRAF6. thereby facilitating downstream activation of NF-κB and MAPK pathways. This regulates inflammatory cytokine production, immune cell activation, and apoptosis. Beyond innate immunity, Pellino 1 is implicated in adaptive immunity, T-cell receptor signaling, and DNA damage response, highlighting its broad regulatory roles.
Pellino 1-specific antibodies are essential tools for studying its expression, post-translational modifications, and interactions in these pathways. Researchers use these antibodies in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore Pellino 1’s tissue distribution, subcellular localization, and dysregulation in diseases. For instance, studies link aberrant Pellino 1 expression to autoimmune disorders, cancers, and neurodegenerative conditions. Antibodies targeting specific epitopes or phosphorylation sites (e.g., Ser-139) further elucidate its activation mechanisms. Commercial Pellino 1 antibodies are typically validated in knockout models to ensure specificity. By enabling precise detection and functional analysis, these antibodies advance understanding of immune regulation and therapeutic targeting of Pellino 1-associated pathologies. (Word count: 249)
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