| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | penta-EF-hand domain containing 1; ABP32; PEF1A |
| Entrez GeneID | 553115 |
| WB Predicted band size | Calculated MW: 30 kDa; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PEF1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PEF1抗体的参考文献示例(注：内容为模拟概括，非真实文献)：
1. **文献名称**：*PEF1 regulates calcium-dependent apoptosis through interaction with ALG-2*
**作者**：Smith J, et al.
**摘要**：通过Western blot和免疫共沉淀实验，利用PEF1抗体证实PEF1与凋亡相关蛋白ALG-2的相互作用，揭示了其在钙离子介导的细胞凋亡中的调控作用。
2. **文献名称**：*Characterization of PEF1 knockout mice reveals its role in neuronal development*
**作者**：Lee S, et al.
**摘要**：使用PEF1抗体进行脑组织免疫组化分析，发现PEF1缺失导致小鼠海马神经元发育异常，提示其参与神经突触形成。
3. **文献名称**：*PEF1 as a novel biomarker in colorectal cancer progression*
**作者**：Wang Y, et al.
**摘要**：通过免疫组化(PEF1抗体)和qPCR技术，证明PEF1在结直肠癌组织中高表达，且与患者生存率负相关。
4. **文献名称**：*Proteomic analysis of PEF1 in cell cycle regulation*
**作者**：Garcia R, et al.
**摘要**：利用PEF1抗体进行流式细胞术和免疫荧光实验，发现PEF1通过调控Cyclin D1影响G1/S期转换，参与细胞周期进程。
建议通过PubMed或Web of Science以“PEF1 antibody”或“PEF1 function”为关键词检索最新文献以获取真实数据。
The PEF1 antibody is designed to target PEF1 (Penta-EF-hand Domain Containing 1), a calcium-binding protein involved in diverse cellular processes. PEF1. also known as PEF-1 or ALG-2-interacting protein 1 (AIP1), belongs to the penta-EF-hand protein family and plays roles in membrane trafficking, apoptosis, and cell cycle regulation. It interacts with proteins like ALG-2 (apoptosis-linked gene 2) to modulate intracellular signaling pathways, particularly those responding to calcium fluctuations. PEF1 is implicated in endoplasmic reticulum (ER)-Golgi transport, endosomal sorting, and maintaining organelle integrity, making it critical for cellular homeostasis.
Antibodies against PEF1 are widely used in research to study its expression, localization, and interactions. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping elucidate PEF1’s functional mechanisms in health and disease. Dysregulation of PEF1 has been linked to cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its potential as a biomarker or therapeutic target. For example, altered PEF1 expression has been observed in certain tumors, suggesting a role in tumor progression or suppression. These antibodies are typically raised in hosts like rabbits or mice and validated for specificity across human, mouse, or rat samples. Ongoing research continues to explore PEF1’s multifaceted roles, with its antibodies serving as essential tools to decode its contributions to cellular physiology and pathology.
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