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产品描述：牛β羟丁酸(βOHB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的β-OHB含量。β-OHB即β-羟基丁酸，是酮体的主要成分之一。它在人体代谢中发挥着重要作用，可作为能量底物供能。研究发现其能通过调节多种信号通路，参与细胞代谢、炎症反应、氧化应激等过程，在神经保护、代谢性疾病等方面研究较多。试剂盒检测范围为50 nmol/mL-800 nmol/mL，适用于畜牧科研领域，例如探究牛群能量代谢特征、营养调控对酮体生成的影响，或作为代谢综合征模型的生物标志物检测工具。实验操作兼容常规实验室设备，为动物营养学、兽医学研究提供高效、稳定的检测方案，数据结果仅用于科学研究，不涉及其他用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-Hydroxybutyric acid, beta-OHB
缩写：β-OHB
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：50 nmol/mL-800 nmol/mL
灵敏度：9.5 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 90-103 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2Average 8000.1360.1380.137 4000.3090.3060.308 2000.6740.6820.678 1001.4701.4751.473 502.7062.7122.709 | ||||||
货期：3-5 working days
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