首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β氨基己糖苷酶A(β-Hex A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的βHex A含量。βHex A是一种重要靶点。它在某些疾病发病机制中起关键作用，如泰-萨克斯病等。研究机制方面，主要聚焦于其在细胞内代谢产物的清除过程，通过解析其结构与功能，试图寻找调节它的方法，以研发治疗相关疾病的药物。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，该产品适用于科研领域中对β - Hex A表达水平的定量分析，可用于细胞模型、动物实验或人体生物样本中酶活性变化的研究，为探索溶酶体贮积症发病机制、代谢通路调控或潜在治疗策略的体外评估提供高效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-hexosaminidase A, beta-Hex A
缩写：βHex A
Uniprot No.：
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 90 | |
| Range % | 85-94 | ||
| 1:2 | Average % | 93 | |
| Range % | 88-94 | ||
| 1:4 | Average % | 105 | |
| Range % | 100-112 | ||
| 1:8 | Average % | 86 | |
| Range % | 80-92 |
回收率：
| The recovery of human β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 1002.8382.9722.9052.782 502.1402.1752.1582.035 251.4161.4061.4111.288 12.50.9030.8950.8990.776 6.250.5160.5020.5090.386 3.120.3300.3530.3420.219 1.560.2640.2810.2730.150 00.1190.1270.123 | ||||||
货期：3-5 working days
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