首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠β内啡肽(β-EP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的β-EP含量。β-内啡肽（β-endorphin）是一种内源性阿片肽，由垂体合成并储存在丘脑、垂体等组织中。它通过作用于μ受体，调节生理活动，并参与成瘾、疼痛和应激反应等生理过程。研究显示，β-EP水平与多种疾病状态相关，如成瘾、疼痛和神经源性炎症等，其具体作用机制尚需进一步研究。试剂盒检测范围为10 pg/mL-2000 pg/mL，该产品可应用于神经生物学研究、疼痛机制探索、应激反应模型评估等科研领域，尤其适合分析不同实验条件下β - EP在体液或组织中的动态变化，为动物模型中的神经递质调控研究提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-Endorphin, beta-EP
缩写：β-EP
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：10 pg/mL-2000 pg/mL
灵敏度：5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of rat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 90 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.2140.2120.213 5000.3650.3360.351 1600.5340.5780.556 400.9390.9940.967 101.4241.4761.450 02.3812.2592.320 | ||||||
货期：3-5 working days
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