| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2 |
| Entrez GeneID | 5164 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PDK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PDK2抗体的3篇示例文献(内容为虚构示例，仅用于参考格式)：
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1. **文献名称**：*"Development of a Monoclonal Antibody Specific to Human PDK2 for Metabolic Studies"*
**作者**：Lee, J. et al.
**摘要**：本研究成功开发了一种针对人PDK2蛋白的高特异性单克隆抗体，通过Western blot和免疫组化验证其在多种细胞系中的灵敏度与特异性，并应用于探究PDK2在癌细胞糖代谢中的调控作用。
2. **文献名称**：*"PDK2 Expression in Diabetic Liver Tissues: Insights from Immunohistochemical Analysis"*
**作者**：Chen, R. & Gupta, S.
**摘要**：利用商业化PDK2抗体，研究者揭示了2型糖尿病小鼠模型中肝脏PDK2蛋白表达的上调现象，提示PDK2在胰岛素抵抗中的潜在作用，抗体特异性通过siRNA敲降实验验证。
3. **文献名称**：*"Role of PDK2 in Neuronal Energy Metabolism: Evidence from Knockout Mice Models"*
**作者**：Martinez, A. et al.
**摘要**：通过PDK2抗体对野生型与敲除小鼠脑组织进行蛋白质分析，发现PDK2缺失导致神经元线粒体丙酮酸脱氢酶活性显著升高，为神经退行性疾病代谢机制提供新线索。
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(注：以上文献为模拟示例，实际引用需查询PubMed或学术数据库获取真实文献。)
The pyruvate dehydrogenase kinase 2 (PDK2) antibody is a crucial tool for studying the regulation of cellular energy metabolism. PDK2 is one of four isoforms (PDK1-4) in the PDK family, which phosphorylates and inhibits the pyruvate dehydrogenase complex (PDC), a key enzyme linking glycolysis to the tricarboxylic acid (TCA) cycle. By inactivating PDC, PDK2 shifts cellular metabolism from oxidative phosphorylation to glycolysis, a phenomenon often observed in cancer (Warburg effect) and metabolic disorders. PDK2 is highly expressed in tissues with dynamic energy demands, such as heart, liver, and skeletal muscle, and its activity is modulated by nutrients, hormones, and hypoxia.
PDK2-specific antibodies enable researchers to investigate the protein's expression, localization, and function in physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study PDK2's role in diseases such as diabetes, obesity, and cancer. For example, elevated PDK2 levels in tumors correlate with poor prognosis, making it a potential therapeutic target. Commercially available PDK2 antibodies are typically validated for specificity (via knockout controls) and cross-reactivity across species (human, mouse, rat). Their applications extend to drug discovery, particularly in testing PDK inhibitors aimed at restoring PDC activity to normalize dysregulated metabolism. Understanding PDK2 dynamics through antibody-based assays contributes to advancing metabolic disease research and targeted therapies.
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