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产品描述：牛β内啡肽(β-EP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的β-EP含量。β - EP即β - 内啡肽，是人体自身产生的具有强大镇痛作用的内源性活性物质。其研究机制主要围绕它与体内阿片受体结合，调节痛觉传导、情绪等生理过程，在镇痛、精神疾病等领域研究较多，对理解人体生理和开发相关疗法有重要意义。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，可为神经生物学、疼痛机制研究或应激反应动物模型提供数据支持，例如探究药物干预、环境刺激对β - EP表达水平的影响；适用于牛源样本的体外定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-Endorphin, beta-EP
缩写：β-EP
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30001.7011.6121.6571.55915001.3761.4581.4171.3197501.1131.1041.1091.0113750.8320.8090.8210.723187.50.5240.5600.5420.44493.750.3670.3590.3630.26546.880.2640.2510.2580.16000.0990.0970.098 | ||||||
货期：3-5 working days
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