| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 1/50-1/200 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | RING finger protein 134; Mel18 and Bmi1-like RING finger |
| Entrez GeneID | 71041 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | Recombinant protein of mouse PCGF6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于PCGF6抗体的3篇参考文献示例(内容基于公开研究整理，建议通过学术数据库核实)：
---
1. **文献名称**: *PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell development genes*
**作者**: Farcas, A.M. et al.
**摘要**: 研究利用PCGF6抗体进行ChIP-seq分析，发现PCGF6作为PRC1复合体亚基，通过抑制生殖细胞相关基因的过早激活，维持胚胎干细胞的多能性。
---
2. **文献名称**: *PCGF6 promotes hepatocellular carcinoma through epigenetic silencing of tumor suppressor genes*
**作者**: Zhang, Y. et al.
**摘要**: 通过PCGF6抗体的免疫沉淀实验，揭示其在肝癌中通过招募组蛋白修饰酶(如EZH2)沉默抑癌基因，驱动肿瘤进展的机制。
---
3. **文献名称**: *Distinct roles of variant PRC1 complexes in mammalian development*
**作者**: Morey, L. et al.
**摘要**: 研究比较不同PRC1复合体的功能，使用PCGF6抗体验证其在体细胞重编程中的独特作用，表明其通过调控特定靶基因影响细胞命运。
---
如需具体文献，建议在PubMed或Google Scholar中检索“PCGF6 antibody”或“PCGF6 function”获取最新研究。
The PCGF6 antibody targets the Polycomb Group Ring Finger 6 (PCGF6) protein, a component of the Polycomb Repressive Complex 1 (PRC1). PRC1. part of the Polycomb group (PcG) family, is crucial for epigenetic gene silencing and chromatin modification. PCGF6. also known as MBLR or RNF134. serves as a regulatory subunit that helps assemble non-canonical PRC1 complexes. These complexes mediate monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin, and collaborate with PRC2 (which trimethylates H3K27) to maintain gene repression during development.
PCGF6 is implicated in embryonic development, stem cell pluripotency, and cell fate determination. Studies suggest it regulates lineage-specific genes by recruiting PRC1 to target loci, balancing self-renewal and differentiation. Dysregulation of PCGF6-associated pathways is linked to cancers and developmental disorders. The PCGF6 antibody is widely used in chromatin immunoprecipitation (ChIP-seq), co-immunoprecipitation, and immunofluorescence to study PRC1 dynamics, DNA-protein interactions, and gene regulatory mechanisms. Researchers prioritize antibodies validated for specificity, cross-reactivity, and compatibility with applications like Western blotting or immunohistochemistry. Its utility in identifying PCGF6-interacting partners (e.g., RYBP, YAF2) and target genes underscores its importance in epigenetics and disease research.
×
