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产品描述:人载脂蛋白H(Apo-H)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的APOH含量。APOH即载脂蛋白 H,可与多种配体结合参与体内多项生理过程。其研究机制包括参与凝血、免疫调节等。它还与多种疾病如系统性红斑狼疮、心血管疾病相关,对其深入研究或可为相关疾病的诊治提供新的思路和靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL,该产品适用于心血管疾病机制研究、脂代谢调控分析、抗磷脂抗体相关病理模型构建等科研领域,为探索Apo - H在血栓形成、氧化应激或免疫调节中的功能提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Activated protein C binding protein ELISA Kit; Activated protein C-binding protein ELISA Kit; Anticardiolipin cofactor ELISA Kit; APC inhibitor ELISA Kit; Apo-H ELISA Kit; APOH ELISA Kit; APOH_HUMAN ELISA Kit; apolipoprotein H (beta-2-glycoprotein I) ELISA Kit; Apolipoprotein H ELISA Kit; B2G1 ELISA Kit; B2GP1 ELISA Kit; B2GPI ELISA Kit; Beta 2 glycoprotein I ELISA Kit; Beta 2 glycoprotein I precursor ELISA Kit; Beta(2)GPI ELISA Kit; Beta-2-glycoprotein 1 ELISA Kit; Beta-2-glycoprotein I ELISA Kit; BG ELISA Kit; Glycoprotein 1; beta-2 ELISA Kit; Glycoprotein I; beta-2 ELISA Kit; OTTMUSP00000003033 ELISA Kit
缩写:APOH
Uniprot No.:P02749
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:15.6 ng/mL-1000 ng/mL
灵敏度:3.9 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human APOH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1000 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2000 | Average % | 102 | ||||
Range % | 97-107 | |||||
1:4000 | Average % | 91 | ||||
Range % | 85-97 | |||||
1:8000 | Average % | 97 | ||||
Range % | 91-103 |
回收率:
The recovery of human APOH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 10002.6382.6892.6642.491 5002.3422.3022.3222.149 2501.6641.6891.6771.504 1251.0711.0981.0850.912 62.50.6720.6340.6530.480 31.20.4240.4050.4150.242 15.60.2820.2790.2810.108 00.1720.1740.173 |
货期:3-5 working days
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