首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β2转铁蛋白(β2TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、cerebrospinalfluid样本中的β2TF含量。试剂盒检测范围为1.25 μg/mL-80 μg/mL，该产品特别适用于神经科学研究领域，可用于探索脑脊液中β2TF水平与血脑屏障功能、神经退行性疾病或颅内感染的相关性，也可用于血清样本中铁代谢异常的机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta 2 transferrin, beta2TF
缩写：β2TF
种属：Homo sapiens (Human)
样本类型：serum, cerebrospinal fluid
检测范围：1.25 μg/mL-80 μg/mL
灵敏度：0.31 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β2TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human β2TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 99-114 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µg/mlOD1OD2AverageCorrected802.9152.9522.9342.769402.2102.0762.1431.978201.5611.6041.5831.418101.0511.0391.0450.88050.6160.6240.6200.4552.50.3960.3690.3830.2181.250.2740.2790.2770.11200.1620.1670.165 | ||||||
货期：3-5 working days
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