| WB | 1/500-1/1000 | Mouse,Rat |
| IF | 1/20 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | HNRPE2; HNRNPE2; hnRNP-E2 |
| Entrez GeneID | 5094 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 35-45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthetic peptide of human PCBP2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇与PCBP2抗体相关的参考文献示例(内容为模拟生成，仅供参考)：
---
1. **文献名称**: *"PCBP2 regulates iron homeostasis through modulation of ferritin mRNA stability"*
**作者**: Chen, L. et al.
**摘要**: 本研究利用PCBP2特异性抗体进行免疫共沉淀和RNA结合实验，揭示PCBP2通过与铁蛋白mRNA的5'非翻译区结合，调控其稳定性并影响细胞铁代谢。Western blot和免疫荧光证实PCBP2在肝细胞中的高表达及其对缺铁应激的响应。
2. **文献名称**: *"Poly(rC)-Binding Protein 2 (PCBP2) interacts with viral RNA to promote enterovirus replication"*
**作者**: Zhang, Y. et al.
**摘要**: 通过免疫沉淀结合质谱分析，发现PCBP2与肠道病毒RNA基因组直接结合，促进病毒复制复合体形成。研究使用PCBP2抗体进行体内外功能缺失实验，证实PCBP2敲低显著抑制病毒复制，提示其作为抗病毒治疗潜在靶点。
3. **文献名称**: *"PCBP2 suppresses tumor metastasis by modulating EGFR mRNA stability in non-small cell lung cancer"*
**作者**: Wang, H. et al.
**摘要**: 该研究通过免疫组化(使用PCBP2抗体)发现PCBP2在肺癌组织中低表达且与不良预后相关。机制研究表明，PCBP2通过结合EGFR mRNA的3'UTR增强其稳定性，从而抑制EMT进程和肿瘤转移。
---
注：以上文献为示例，实际引用需查询PubMed或Web of Science等数据库获取真实发表论文。如需具体文献协助，可提供更详细的研究方向或应用场景。
The PCBP2 antibody is a crucial tool for studying the poly(rC)-binding protein 2 (PCBP2), a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. PCBP2. also known as αCP2 or hnRNP-E2. is a multifunctional RNA-binding protein involved in transcriptional and post-transcriptional gene regulation. It plays roles in mRNA stability, splicing, translation initiation, and iron metabolism by binding to poly(rC)-rich sequences in target RNAs. PCBP2 is implicated in viral replication, cell proliferation, apoptosis, and stress responses, with links to cancers, neurodegenerative diseases, and viral infections (e.g., hepatitis C virus).
PCBP2 antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to detect PCBP2 expression and localization. These antibodies are typically raised in rabbits or mice, targeting specific epitopes within PCBP2’s KH domains or variable regions. Both monoclonal and polyclonal variants exist, offering trade-offs between specificity and sensitivity. Validated antibodies show minimal cross-reactivity with homologs like PCBP1. ensuring accurate detection in tissues or cell lines (e.g., HeLa, HEK293).
Commercial PCBP2 antibodies are available from suppliers like Santa Cruz Biotechnology, Abcam, and Cell Signaling Technology. Researchers prioritize antibodies with robust validation data (e.g., knockout controls, application-specific citations) to ensure reproducibility in experimental models ranging from cancer (e.g., hepatocellular carcinoma) to neurological studies. Proper antibody selection is critical for elucidating PCBP2’s context-dependent roles in disease mechanisms and cellular homeostasis.
×
