| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAK2; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58 |
| Entrez GeneID | 5062 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PAK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与PAK2抗体相关的文献及其摘要概括：
1. **文献名称**: "p21-Activated Kinase 2 (PAK2) Is Required for Human Neutrophil Degranulation"
**作者**: Allen L. et al.
**摘要**: 本研究通过特异性PAK2抗体发现PAK2在中性粒细胞脱颗粒过程中的关键作用，抑制PAK2会显著降低溶酶体酶释放，表明其在免疫反应中的调控功能。
2. **文献名称**: "Selective Activation of PAK2 by Fas Stress and Death Signals"
**作者**: Rudel T. et al.
**摘要**: 利用PAK2特异性抗体揭示PAK2在Fas配体诱导的细胞凋亡信号通路中被激活，其剪切形式(p34 PAK2)与caspase介导的细胞死亡直接相关。
3. **文献名称**: "PAK2 Phosphorylation and Regulation of Endothelial Nitric Oxide Synthase"
**作者**: Lee S. et al.
**摘要**: 通过PAK2抗体检测发现，PAK2磷酸化内皮型一氧化氮合酶(eNOS)，调控血管内皮功能，为心血管疾病治疗提供潜在靶点。
4. **文献名称**: "Antibody-Based Profiling of p21-Activated Kinases in Cancer"
**作者**: Kumar R. et al.
**摘要**: 研究开发了高特异性PAK2抗体，用于分析多种癌症组织中PAK2的表达水平，发现其过表达与肿瘤侵袭性及预后不良显著相关。
这些文献覆盖了PAK2抗体在功能研究、信号通路分析及疾病关联中的应用场景。
The p21-activated kinase 2 (PAK2) is a serine/threonine kinase belonging to the PAK family, which comprises six members divided into two groups (Group I: PAK1-3; Group II: PAK4-6). PAK2 plays critical roles in regulating cytoskeletal dynamics, cell motility, survival, and apoptosis. It is activated by binding to Rho-family GTPases, such as Cdc42 and Rac, which disrupt its auto-inhibitory conformation, enabling kinase activity. PAK2 is unique among Group I PAKs due to its dual role in pro-survival and pro-apoptotic signaling. While basal PAK2 activity supports cell proliferation and migration, caspase-mediated cleavage during stress generates a constitutively active fragment that promotes apoptosis.
PAK2 antibodies are essential tools for studying its expression, activation, and localization in various cellular contexts. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate PAK2's involvement in diseases, including cancer, neurodegenerative disorders, and immune dysregulation. For example, PAK2 dysregulation has been linked to tumor metastasis and resistance to therapy, making it a potential therapeutic target. These antibodies often target specific regions, such as the kinase domain or caspase cleavage sites, to distinguish between full-length and activated forms. Researchers also utilize phospho-specific antibodies to detect activation-loop phosphorylation (e.g., Thr402), a marker of PAK2 activity. Understanding PAK2's multifaceted roles continues to advance insights into cell signaling and disease mechanisms.
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