| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1 |
| Entrez GeneID | 142 |
| WB Predicted band size | Calculated MW: 113 kDa; Observed MW: 116,89 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PARP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于PARP1抗体的代表性文献，包含文献名称、作者和摘要概括：
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1. **文献名称**：*Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase*
**作者**：Bryant, H.E., et al.
**摘要**：该研究首次提出PARP抑制剂对BRCA缺陷肿瘤的选择性杀伤作用。实验中使用PARP1抗体通过Western blot检测PARP1蛋白的切割(cleavage)，证明PARP抑制导致DNA损伤积累并诱导肿瘤细胞凋亡。
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2. **文献名称**：*PARP1-dependent kinetics of recruitment of MRE11 and NBS1 proteins to multiple DNA damage sites*
**作者**：Haince, J.F., et al.
**摘要**：研究通过免疫荧光和PARP1抗体定位，揭示了PARP1在DNA损伤修复中招募MRE11/NBS1复合物的动态过程，阐明了PARP1在DNA断裂位点的功能依赖性招募机制。
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3. **文献名称**：*BMN 673. a novel and highly potent PARP1/2 inhibitor for the treatment of human cancers with DNA repair deficiency*
**作者**：Shen, Y., et al.
**摘要**：该研究开发了新型PARP抑制剂BMN 673.并利用PARP1抗体进行免疫组化(IHC)和流式细胞术，验证了药物对PARP1酶活的抑制作用及其在DNA修复缺陷癌细胞中的疗效。
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**备注**：以上文献均发表于高影响力期刊(如*Nature*、*Cancer Research*)，涉及PARP1抗体的应用场景包括蛋白表达检测(Western blot)、亚细胞定位(免疫荧光)及药效评估(IHC)。如需具体出版年份或补充文献，可进一步提供。
PARP1 (Poly(ADP-ribose) polymerase 1) is a nuclear enzyme critical for DNA repair, genomic stability, and cellular response to damage. It catalyzes the transfer of ADP-ribose units to target proteins, a post-translational modification involved in DNA repair processes, particularly base excision repair. PARP1 also regulates transcription, chromatin remodeling, and apoptosis. Dysregulation of PARP1 is linked to cancer, neurodegenerative diseases, and inflammation.
PARP1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blot, immunohistochemistry, and immunofluorescence to detect PARP1 in tissues or cultured cells. These antibodies help identify PARP1 cleavage (a hallmark of apoptosis) or hyperactivation (indicative of DNA damage). In cancer research, PARP1 antibodies aid in evaluating PARP inhibitor sensitivity, as drugs like olaparib target PARP1 to exploit synthetic lethality in BRCA-mutated cancers. Additionally, PARP1 expression levels are explored as potential biomarkers for therapeutic response or prognosis.
Recent studies also investigate PARP1's role in non-oncological contexts, such as stroke and oxidative stress. Validating antibody specificity remains crucial, given PARP1’s homology with other PARP family members. Overall, PARP1 antibodies are pivotal in advancing both basic research and clinical applications.
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